This invention relates to the improved hydrocarbon recovery using an enzyme-based green solvent in unconventional reservoir

Enzymes modify solid-to-liquid interactions, therefore having more impact on the rock surface than the oil–water interface. The de-adhesion of crude oil from the rock is related to the catalytic effect of the enzymes.

Enzymes are biological catalysts that expedite reactions. Naturally, all proteins have surface activity potential.

In this paper, we review the progress in understanding enzyme applications in hydrocarbon production, drawn from extensive experimental and field studies.

Biological enzymes has the potential to improve this recovery factor up to 25%. Moreover, the effect of enzymes is everlasting

GreenZyme® interacts naturally with formation salts, using reservoir salinity to strengthen its polarity‑changing effect on the oil–rock interface. Instead of losing efficiency in high‑TDS environments, the enzyme creates synergistic behavior that improves oil detachment, mobility, and overall recovery in high‑salinity reservoirs.

A microfluidics study of enzyme-enhanced oil recovery (EEOR), showing how lowering interfacial tension and altering rock wettability mobilizes capillary-trapped oil, with lab and field studies reporting up to 16% incremental recovery.