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GreenZyme® single well booster treatment

Treatments are usually very simple and can be completed in a single day. Most Huff n Puff treatments to date have used GreenZyme® concentrate diluted to solutions between 2%-10% with over 90% success rate world wide.
The concentrate is mixed with available/compatible water at the site to then pump inside the well using traditional injection methods. The objective is to displace the solution into the oil producing zone.
From here GreenZyme® will diffuse further in the layer making contact with the rock to create the positive skin that will help mobilize crude oil. Additional formation water is injected to clear the injection tube length and displace the remaining fluid mix into the formation

Water based enzyme solution can also be pre-heated at the time of well treatment injection to 80C degrees to avoid pour point issues, and has been recorded active and working at to 270 C in laboratory testing.

Water based solution compatible with multiple oil well intervention tactics, different well profiles and field challenges.

Once pumping is completed, the well is soaked for 3-5 days to give the enzyme fluid time to diffuse in the producing layer, promoting changes in wettability by the creation of the enzyme-water skin on the rock surface. GreenZyme molecules will produce a change of adhesion behavior of hydrocarbons that will increase reservoir fluid mobility.

Like a detergent, the result of using GreenZyme® in any reservoir is increased permeation of the reservoir fluid thru rock by washing away of oil from rock.
Before opening the oil well to resume production a change in static well fluid is the expected effect of GreenZyme® action indicating more fluid is available around the well bore to be produced.  

aeco sacia mobie crude oil with GreenZyme Bio agent fluid

Effective and proven solution for:

In Heavy Oil for fingering and water cone mitigation, Subsurface emulsion dispersant,
Unproduced oil pockets, New wells with rapid decline.

​  Features

  • Cost Effective oil production enhancer

  • Easy to implement with available field equipment

  • Water based oil separator additive

  Compatible with:
   Vertical, Horizontal & Off-shore wells

  1. Huff n Puff 

  2. Waterflooding & Cluster Floods

  3. WAG, Steam & SAGD pre-soak

  Implemented using:

  • Surface injection pumps / truck

  • Coiled Tubing equipment

  • Bullheading,

  • with completion fluids

GreenZyme®  Smart Water Additive

The water based Enzyme Fluid Technology is a perfect additive to any water process operation. GreenZyme® is designed as a hydrocarbon separator that turns water into a powerful detergent surfactant that dissipates crude oil from any surface. 

It can easily be used in reservoir oil production when mixed into injection water drive. Enzyme Enhanced Oil Recovery (EEOR) treatments have shown the use of enzyme detergent can decrease injection pressure of the water drive, increase capacity of the formation to receive water, improve sweep efficiency and volume recovery. 

GreenZyme® penetrates the pores to displace crude oil.


Biologically made yet inert, GreenZyme® is not a solvent and does not react with other chemicals nor the rock. Positive effect of enzyme fluid is that promotes a polar effect in the fluid environment and the rock substrate using them to increase the oil liberation effect. GreenZyme® has the ability to perform oil liberation in high salinity environments.


Combining GreenZyme® water based technology with Steam cycles and SAGD has been found to be a very effective way to use the technology as the oil is more mobile with temperature. Enzyme molecules adsorbed to the rock surface do not denature with temperature, therefore will promote crude mobility creating non attachment surfaces in the drainage zone.


The synergy is created when enzyme technology penetrates and diffuses in the formation changing the attachment behavior of oil, improving permeability and fluid mobility in the reservoir at the time that is sweeped by the steam. Optimization report have shown 25% to 50% less steam is required for Steam Cycle with an incremental increase in recovery. 

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GreenZyme®  for waterflood & Clusters

The GreenZyme® Enhanced Oil Recovery (GEOR) fluid technology can be slugged or injected sporadically in a waterflooding to improve the conditions of the sweep in the formation to promote more oil liberation in its path, mobilize unproduced skipped oil, mitigate fingering and reach further into the unproduced oil pockets.


Pumping GreenZyme® into injector wells is a simple procedure. Given the limits that currently exist for recovery technologies, treatment with enzyme fluid solution is reliable and cost effective way to achieve improved oil recovery, including at lower API gravity oil.


Clusters can be treated from central injector to produce a multi-well effect, which creates the opportunity to use less product to reach more wells. GreenZyme® molecule is highly diffusible in water and will travel in the formation liberating oil in its path while displacing it into the near by wells.

greenzyme bergen waterflood thesis crude sweep university study

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Container & packing


  • 220 gallon IBC tote with integrated pallet

    • 55gal drum + wood pallet (4 drums per pallet)

Cargo is inspected before it boards the shipping container:

• IBC or Drum & Pallet Integrity.

• Sealed Top Cap with additional tamper evident seals

• Steel Strapping for drums, steel cage for IBC protection

• Secured Bottom IBC Valve with tamper evident seal

Quality Control & Inventory Tracking to each individual country:

• Proper Import - Export labeling is attached to each Drum or IBC.

• Each Drum or IBC of GreenZyme® carries a Unique Numbered Security Identification Label specific to each Country or Territory

Certificate of Shipping, Transport & Free Trade Agreements:

• Shipping Container Number

• Quality Control Identification Label Numbers

• Shipping Container Metallic Seal Number

• Project Name and Destination


Biotech Processing Supply works with agents and Oil Field operators to arrange project Evaluation, Engineering, Promotion, Trade, Distribution, Shipment and Implementation of GreenZyme® Technology

Most of our orders ship in 20’ or 40’ containers using FOB or CIF modality for international shipping, flat bed trucks for distribution in the Continental USA to client or Forwarding agent.

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